[Studies on fermentation of decane 1,10-dicarboxylic acid(DC12)]

Candida tropicalis strain UH-2-48 can increase the production of decane 1,10-dicarboxylic acid(DC12) yield by 30% (from 81.7 g/L to 108.2 g/L) after regulating the fermentation condition. The purity of the DC12 is above 97%, which can meet the criteria of the succeeding synthesized industrial productions. Emulsifier can not only destroy the structure of the cell membrane in some degree, but also can enhance the permeability of the cell membrane. Furthermore, the structure of the emulsifier itself can also effect its function. Tween 60(0.10%) can enhance the production of DC12 tremendously. Urea, as the nitrogen resource, can affect the activity of cytochrome P450 enzyme. Some biochemistry and biophysics factors, such as penicillin, vitamin B2 and alanine, can increase the yield of DC12 during the process of fermentation. Ferment in 20 m3 fermentator, under the optimal condition, the average yield of DC12 using the strain HP-12 which derived from UH-2-48, is 202.1 g/L.