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Collagenolytic protease preparations from invertebrates: experimental study of acute and chronic toxicity
B A Paramonov1, O A Klimova, V Y Chebotarev
1St. Petersburg-Technologiya, St. Petersburg.
Abstract:
Toxicity of active matter of new structure-destroying enzyme preparations containing a complex of collagenolytic proteases from invertebrates (Collagenase from hydrobionts, Polycollagenase-K, Fermenkol) was studied. The absence of acute toxicity in skin application is proved, and LD(50) for intraperitoneal administration to mice and rats were determined. A long-term treatment with clinical doses under various types of application does not affect body weight gain, biochemical indices of the plasma, ECG, diuresis, and urine content.
Insights
New enzyme preparations containing invertebrate collagenolytic proteases show no acute toxicity via skin application. Long-term studies in mice and rats confirmed safety, with no adverse effects on key physiological and biochemical markers.
Area of Science:
- Biochemistry
- Pharmacology
- Toxicology
Background:
- Structure-destroying enzyme preparations utilize invertebrate collagenolytic proteases.
- Key preparations include Collagenase from hydrobionts, Polycollagenase-K, and Fermenkol.
Purpose of the Study:
- To evaluate the toxicity of novel enzyme preparations containing invertebrate collagenolytic proteases.
- To determine the safety profile of these enzyme preparations for potential therapeutic applications.
Main Methods:
- Acute toxicity testing via skin application.
- Determination of LD(50) values for intraperitoneal administration in mice and rats.
- Long-term administration studies assessing body weight, plasma biochemistry, ECG, diuresis, and urine content.
Main Results:
- Absence of acute toxicity was demonstrated for skin application.
- LD(50) values were established for intraperitoneal routes in rodents.
- No significant adverse effects were observed during long-term treatment with clinical doses across various application methods.
Conclusions:
- The studied enzyme preparations containing invertebrate collagenolytic proteases exhibit a favorable safety profile.
- These findings support the potential use of these enzyme preparations in applications requiring structure-destroying enzymatic activity without significant toxicological concerns.
