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Related Experiment Videos

Productivity enhancement in l-sorbose fermentation using oxygen vector.

Giridhar1, Srivastava

  • 1Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, 110016, New Delhi, India

Enzyme and Microbial Technology
|September 9, 2000
PubMed
Summary

N-hexadecane enhances sorbose production from sorbitol by Acetobacter suboxydans. This oxygen vector improves bioconversion efficiency, increasing sorbose accumulation and productivity in fermentation processes.

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Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Microbial Fermentation

Background:

  • Sorbose is a key intermediate in Vitamin-C synthesis.
  • Acetobacter suboxydans facilitates the bioconversion of sorbitol to sorbose.
  • Optimizing fermentation conditions is crucial for efficient sorbose production.

Purpose of the Study:

  • To investigate the efficacy of N-hexadecane as an oxygen vector in enhancing sorbose accumulation and productivity.
  • To evaluate the impact of varying N-hexadecane concentrations on the sorbitol-to-sorbose bioconversion process.
  • To determine the effect of N-hexadecane on batch and fed-batch fermentation parameters.

Main Methods:

  • Shake flask fermentations with varying N-hexadecane concentrations (1-6% v/v).
  • Batch and fed-batch fermentation experiments utilizing Acetobacter suboxydans.

Related Experiment Videos

  • Measurement of sorbose accumulation, productivity, and process time.

    • Main Results:

    • Addition of 1-4% N-hexadecane significantly increased sorbose accumulation compared to controls.
    • Maximum sorbose accumulation reached 82.12 kgm(-3) in 24 h with 4% N-hexadecane.
    • Batch fermentation time decreased from 14 to 12 h, and productivity increased from 14.3 to 16.7 kgm(-3)h(-1) with 4% N-hexadecane.
    • Fed-batch fermentation with 4% N-hexadecane yielded 311.68 kgm(-3) sorbose in 20 h, with a productivity of 15.9 kgm(-3)h(-1).

    Conclusions:

    • N-hexadecane acts as an effective oxygen vector, improving sorbose production efficiency.
    • Optimal N-hexadecane concentration enhances both sorbose yield and fermentation productivity.
    • The use of N-hexadecane offers a viable strategy for optimizing industrial sorbose bioconversion.