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[Characteristics of capsular bacteria, isolated from children with acute intestinal diseases]
Insights
This study investigated Klebsiella in young children with gastrointestinal issues, finding it in nearly 40% of patients. Researchers characterized Klebsiella strains, noting variations in biochemical properties and identifying specific antigens and fimbriae.
Area of Science:
- Microbiology
- Pediatrics
- Infectious Diseases
Background:
- Conditionally pathogenic Enterobacteriaceae, particularly Klebsiella, are implicated in pediatric gastrointestinal disorders.
- Understanding the prevalence and characteristics of Klebsiella in children is crucial for diagnosing and managing infections.
Purpose of the Study:
- To determine the prevalence of Klebsiella in young children with gastroenterocolitis, dyspepsia, and food poisoning.
- To characterize the morphological, biochemical, and antigenic properties of isolated Klebsiella strains.
Main Methods:
- Microscopic examination for capsules.
- Biochemical property determination using Kaufman's method.
- Antigenic structure analysis, including K-antigen identification.
- Detection of fimbriae and bacteriocines.
Main Results:
- Klebsiella was isolated from 39.8% of 256 children with gastrointestinal issues and 22.3% of 67 healthy children.
- 308 Klebsiella strains were analyzed for morphological, biochemical, and antigenic properties.
- K-antigen was found in 185 strains (99 Kl. pneumoniae, 86 Kl. aerogenes).
- Variations from typical biochemical patterns were observed in some strains.
Conclusions:
- Klebsiella is a significant finding in pediatric gastrointestinal illnesses.
- Characterization of Klebsiella strains reveals diversity in biochemical profiles and antigenic structures.
- Further research into Klebsiella virulence factors like fimbriae and bacteriocines is warranted.
Abstract:
A study was made of 256 young children suffering from gastroenterocolitis, dyspepsia, food poisoning for the presence of conditionally pathogenic representatives of the Enterobacteriaceae family. Klebsiellae were isolated in 39.8% of the patients and in 22.3% of 67 healthy children. A study was made of morphological, cultural-biochemical properties and the antigenic structure in 308 strains of the Klebsiella denus. Capsules were detected by microscopic examination. Biochemical properties were determined after Kaufman, and some of the cultures proved to differ from the typical biochemical pattern described by this author. K-antigen was found in 185 klebsiella strains; of this number 99 strains belonged to Kl. pneumoniae and 86--to Kl. aerogenes. Fimbria were revealed in some of the Kl. pneumoniae and Kl. aerogenes strains. Bacteriocines were detected in 103 of 206 strains (by Abbot and Shennon's method).