Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple semisolid subtraction method using carbodiimide-coated microplates.

J Kikuchi1, Y Furukawa, O Suzuki

  • 1Division of Molecular Hematopoiesis, Jichi Medical School, Tochigi, Japan.

Molecular Biotechnology
|September 15, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Erratum: Centrality-Dependent Modification of Jet-Production Rates in Deuteron-Gold Collisions at sqrt[s_{NN}]=200  GeV [Phys. Rev. Lett. 116, 122301 (2016)].

Physical review letters·2025
Same author

Successful mild brain hypothermia therapy followed by targeted temperature management for pediatric hemorrhagic shock and encephalopathy syndrome.

European review for medical and pharmacological sciences·2021
Same author

Beam Energy and Centrality Dependence of Direct-Photon Emission from Ultrarelativistic Heavy-Ion Collisions.

Physical review letters·2019
Same author

Post-occlusional hyperemia for fractional flow reserve assessment and pull-back curve analysis.

Cardiovascular intervention and therapeutics·2019
Same author

Centrality-Dependent Modification of Jet-Production Rates in Deuteron-Gold Collisions at √[s(NN)]=200 GeV.

Physical review letters·2016
Same author

Measurements of Elliptic and Triangular Flow in High-Multiplicity 3He+Au Collisions at √(s(NN))=200 GeV.

Physical review letters·2015

This study introduces a new carbodiimide-based method for enriching specific gene transcripts. The technique efficiently isolates target-specific genes by removing common ones, aiding in the study of rare transcripts.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Gene expression analysis requires methods to isolate specific transcripts.
  • Current techniques may lack efficiency in enriching rare or specific gene populations.

Purpose of the Study:

  • To develop a novel and efficient subtraction method for enriching target-specific nucleic acid sequences.
  • To optimize conditions for a carbodiimide-mediated hybridization technique.

Main Methods:

  • Utilized carbodiimide-bound microplates for nucleic acid hybridization.
  • Developed a semisolid phase hybridization between driver RNA and target cDNA.
  • Optimized target/driver RNA ratio (1:10) and hybridization time (24 hours).

Main Results:

Related Experiment Videos

  • Successfully enriched target-specific genes by removing common RNA/cDNA hybrids.
  • Demonstrated effective enrichment of rare transcripts.
  • Showcased the method's applicability through the isolation of megakaryocyte-specific genes.

Conclusions:

  • The carbodiimide-based subtraction method is simple, efficient, and effective for enriching specific gene transcripts.
  • This technique offers advantages in monitoring hybridization efficiency and isolating rare transcripts.
  • The method is broadly applicable to molecular biology and genetics research for identifying cell- or tissue-specific genes.