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GLUT-3 expression in human skeletal muscle.

C A Stuart1, G Wen, B H Peng

  • 1Department of Internal Medicine, The University of Texas Medical Branch at Galveston, Galveston, Texas 77555, USA. cstuart@utmb.edu

American Journal of Physiology. Endocrinology and Metabolism
|September 23, 2000
PubMed
Summary

Glucose transporter 3 (GLUT-3) is present in human skeletal muscle fibers, particularly in slow-twitch types. GLUT-3 protein is mainly located within the triads and transverse tubules of muscle cells.

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Area of Science:

  • Muscle physiology
  • Cellular biology
  • Neuroscience

Background:

  • The precise localization of Glucose transporter 3 (GLUT-3) within muscle tissue was previously undetermined.
  • Understanding GLUT-3's location is crucial for comprehending glucose uptake mechanisms in skeletal muscle.

Purpose of the Study:

  • To elucidate the cellular and subcellular localization of GLUT-3 mRNA and protein in human skeletal muscle.
  • To investigate the presence of GLUT-3 in associated nerve tissues.

Main Methods:

  • In situ hybridization to detect GLUT-3 mRNA.
  • Immunofluorescence and electron microscopy with specific antibodies to localize GLUT-3 protein.
  • NADH-tetrazolium reductase staining to differentiate muscle fiber types.
  • Electron microscopy of human peripheral nerve.
Keywords:
NASA Discipline Regulatory PhysiologyNon-NASA Center

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Main Results:

  • GLUT-3 mRNA and protein are expressed in all human skeletal muscle fibers.
  • Slow-twitch muscle fibers exhibit higher GLUT-3 protein levels than fast-twitch fibers.
  • GLUT-3 protein is predominantly localized to triads and transverse tubules within muscle fibers.
  • GLUT-3 is found within the axon and myelin of human peripheral nerves, associated with mitochondria.

Conclusions:

  • GLUT-3 is widely expressed in human skeletal muscle, with preferential localization in slow-twitch fibers.
  • The protein's location within triads and transverse tubules suggests a role in muscle glucose transport.
  • GLUT-3 expression in peripheral nerves indicates potential roles beyond skeletal muscle glucose metabolism.