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Purification of alkaline phosphatase from chicken intestine by expanded-bed affinity chromatography on dye-linked
1Chemistry Department, Indian Institute of Technology, Hauz Khas, New Delhi 110 016, India.
Biotechnology and Applied Biochemistry
|September 26, 2000
Abstract:
Alkaline phosphatase from chicken intestine was purified on dye-linked cellulose beads. The various dye-linked cellulose beads were screened in the batch mode for purification of the enzyme and the promising dyes were tried in the expanded-bed mode. The one-step expanded-bed affinity chromatography on Cibacron Blue-linked cellulose beads yielded 48-fold purification with an activity recovery of 70%.