Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Basic studies on group B streptococcal (GBS) culture medium.

K Higashide1, Y Keduka, Y Tanaka

  • 1Chita Municipal Hospital, 2-1 Nagai, Shinti, Tita 478-0017, Japan.

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai Shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology
|September 27, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A 7.6kb DNA region from Streptomyces kasugaensis M338-M1 includes some genes responsible for kasugamycin biosynthesis.

The Journal of antibiotics·1998
Same author

How to decrease false-positive cases of ovarian cancer screening by transvaginal sonography.

Journal of experimental & clinical cancer research : CR·1997
Same author

Establishment and characterization of acquired resistance to platinum anticancer drugs in human ovarian carcinoma cells.

Japanese journal of cancer research : Gann·1995
Same author

Differential expression of VLA-alpha 4 and VLA-beta 1 discriminates multiple subsets of CD4+CD45R0+ "memory" T cells.

Journal of immunology (Baltimore, Md. : 1950)·1992
Same author

Thymic depletion and peripheral activation of class I major histocompatibility complex-restricted T cells by soluble peptide in T-cell receptor transgenic mice.

Proceedings of the National Academy of Sciences of the United States of America·1992
Same author

Eating disorder and schizophrenia.

The Japanese journal of psychiatry and neurology·1992
Same journal

[Evaluation of the Utility of 72-hour Cultivation in Vancomycin-resistant Enterococci (VRE) Screening Medium].

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology·2026
Same journal

[A Case of Necrotizing Fasciitis Caused by <i>Campylobacter fetus</i> subsp. <i>fetus</i>].

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology·2026
Same journal

[Clinical Evaluation of BacT/ALERT VIRTUO Blood Culture System for Detection of Anaerobic Bacteria].

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology·2026
Same journal

[Evaluation of Bacterial Identification Performance Using the I-dOne Measuring Device Based on the Principle of the ATR-FTIR Method].

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology·2024
Same journal

[Initiatives for Rapid Reporting of Antibiotic Resistance Information Using Disk Diffusion for Positive Blood Culture].

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology·2024
Same journal

[Evaluation of Quick Chaser CD GDH/TOX, a Rapid Diagnostic Kit for Simultaneous Detection of <i>Clostridioides difficile</i> Antigen and Toxin A/B, in Fecal Specimens].

Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology·2024
See all related articles

The study found that a basic culture medium without yeast extract or serum is best for detecting Group B Streptococcus (GBS) pigmentation. This basic medium demonstrated the strongest and quickest pigmentation results for GBS detection.

Area of Science:

  • Microbiology
  • Bacteriology

Background:

  • Group B Streptococcus (GBS) pigmentation is traditionally thought to require yeast extract and serum.
  • The necessity of these components for GBS pigmentation has not been thoroughly investigated.

Purpose of the Study:

  • To evaluate the necessity of yeast extract and serum for Group B Streptococcus (GBS) pigmentation.
  • To determine the optimal culture medium for GBS detection based on pigmentation.

Main Methods:

  • Four culture media were tested: Basic medium (serum-excluded), S medium (New Granada), Y medium (Basic + yeast extract), and SY medium (Basic + serum + yeast extract).
  • Pigmentation potency and initiation speed were assessed for 27 GBS strains across 7 serotypes.
  • The relationship between GBS quantity and positive detection ratio was analyzed for each medium.

Related Experiment Videos

Main Results:

  • The Basic medium exhibited the strongest pigmentation potency and the quickest initiation of pigmentation.
  • S medium and SY medium showed the weakest pigmentation potency and slowest initiation.
  • No significant differences were observed in the GBS quantity-to-detection ratio across the tested media.

Conclusions:

  • The exclusion of yeast extract and serum enhances, rather than hinders, GBS pigmentation.
  • The Basic medium is identified as the most effective for GBS detection due to superior pigmentation characteristics.
  • These findings challenge conventional assumptions regarding GBS culture media requirements.