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Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
Non-aqueous capillary electrophoresis
M L Riekkola1, M Jussila, S P Porras
1Department of Chemistry, University of Helsinki, Finland. marja-liisa.riekkola@helsinki.fi
Journal of Chromatography. A
|October 25, 2000
Summary
Non-aqueous capillary electrophoresis offers enhanced separation selectivity and detection sensitivity by utilizing diverse organic solvents. This technique allows for higher electric fields and larger sample loads, expanding its analytical potential.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Capillary electrophoresis (CE) is a powerful separation technique.
- Traditional aqueous CE has limitations in selectivity and sensitivity.
- Non-aqueous solvents offer unique physicochemical properties for CE.
Purpose of the Study:
- To explore the benefits and potential of non-aqueous capillary electrophoresis (NACE).
- To demonstrate the feasibility of NACE across various applications.
Main Methods:
- Utilizing a wide selection of organic solvents with diverse properties.
- Employing high electric field strengths due to lower currents.
- Using wide bore capillaries for increased sample loading.
Main Results:
- Achieved enhanced separation selectivity through solvent manipulation.
- Demonstrated improved detection sensitivity in many cases.
- Facilitated larger sample loads with wider capillaries.
Conclusions:
- Non-aqueous capillary electrophoresis significantly broadens the scope of CE.
- NACE is a versatile and feasible technique with demonstrated applications.
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