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Glutathione reductase in wheat grain. 1. Isolation and characterization.

F de Lamotte1, N Vianey-Liaud, M P Duviau

  • 1Unité de Biochimie et Biologie Moléculaire des Céréales, INRA, 2 place Viala, 34060 Montpellier Cedex 01, France.

Journal of Agricultural and Food Chemistry
|October 29, 2000
PubMed
Summary
This summary is machine-generated.

A single form of glutathione reductase (GR) was identified in mature durum wheat kernels, appearing after 18 days and persisting through grain development. This GR enzyme showed stability across various temperatures and pH levels.

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Area of Science:

  • Biochemistry
  • Plant Physiology
  • Molecular Biology

Background:

  • Glutathione reductase (GR) plays a crucial role in cellular redox homeostasis.
  • Understanding GR isoforms in crop plants like durum wheat is vital for optimizing grain development and stress tolerance.

Purpose of the Study:

  • To isolate and characterize the glutathione reductase (GR) enzyme present in mature durum wheat (Triticum durum) kernels.
  • To investigate the developmental expression pattern of GR isoforms during grain filling.

Main Methods:

  • Enzyme isolation and purification from mature durum wheat endosperm.
  • Characterization of enzyme kinetics (K(m), V(m)) and physical properties (molecular mass, pI).
  • Analysis of GR isoform presence during different stages of grain development post-anthesis.

Main Results:

  • A single GR isoform was detected in mature durum wheat kernels, appearing around 18 days post-anthesis.
  • Another GR isoform, present in early development, disappeared between 20-30 days post-anthesis.
  • The mature GR enzyme consists of two 60 kDa monomers, with specific kinetic parameters (K(m) for NADPH: 3.7 µM, GSSG: 9.1 µM) and optimal activity at pH 7.5-8.0.
  • The enzyme exhibited significant stability at high and low temperatures and an isoelectric point between pH 4.4-4.5.

Conclusions:

  • The study identified and characterized a specific glutathione reductase (GR) isoform crucial for mature durum wheat grain development.
  • The developmental regulation and biochemical properties of this GR enzyme suggest its importance in maintaining redox balance during later stages of grain filling.
  • The enzyme's stability indicates potential for resilience in varying environmental conditions affecting wheat growth.