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Related Concept Videos

Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.

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Related Experiment Video

Updated: Jul 26, 2026

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant
08:52

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant

Published on: May 27, 2011

Virus-mediated gene transfer for cutaneous gene therapy.

S Ghazizadeh1, L B Taichman

  • 1Department of Oral Biology and Pathology, SUNY at Stony Brook, Stony Brook, NY 11794, USA.

Human Gene Therapy
|November 21, 2000
PubMed
Summary

Cutaneous gene therapy uses viral vectors for gene transfer directly into skin cells. This approach leverages accessible skin and susceptible keratinocyte stem cells for potential therapeutic applications.

Area of Science:

  • Dermatology
  • Gene Therapy
  • Molecular Biology

Background:

  • The skin acts as a barrier, limiting traditional gene therapy delivery methods.
  • Cutaneous gene therapy presents unique challenges and opportunities for viral vector applications.

Purpose of the Study:

  • To explore the potential and limitations of viral vectors for gene transfer in the skin.
  • To evaluate the feasibility of using keratinocyte stem cells for cutaneous gene therapy.

Main Methods:

  • Investigating the susceptibility of skin's keratinocyte stem cells to in vivo retroviral vector transduction.
  • Assessing the persistence and transgene expression of cultured, transduced keratinocyte stem cells grafted onto immunocompromised mice.

Main Results:

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Published on: August 12, 2014

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium
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Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Published on: August 7, 2015

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Last Updated: Jul 26, 2026

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Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant

Published on: May 27, 2011

Vascular Gene Transfer from Metallic Stent Surfaces Using Adenoviral Vectors Tethered through Hydrolysable Cross-linkers
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Vascular Gene Transfer from Metallic Stent Surfaces Using Adenoviral Vectors Tethered through Hydrolysable Cross-linkers

Published on: August 12, 2014

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium
06:48

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Published on: August 7, 2015

  • Skin's physical barrier and vascular structure pose delivery challenges.
  • Keratinocyte stem cells are amenable to direct in vivo and in vitro gene transfer.
  • Transduced keratinocyte stem cells demonstrate persistence and expression after grafting.

Conclusions:

  • Virus-mediated gene transfer is a primary strategy for cutaneous gene therapy.
  • Keratinocyte stem cell manipulation offers a viable route for skin gene therapy.
  • Further research into viral vectors is crucial for advancing skin-targeted therapies.