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Determination of nociceptin-orphanin FQ metabolites by capillary LC-MS
P Suder1, J Kotlińska, A Legowska
1Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Krakow, Poland.
Brain Research. Brain Research Protocols
|November 22, 2000
Abstract:
Nociceptin-orphanin FQ (OFQ/N) is a newly discovered peptide involved in pain transmission. The method is described to identify metabolic pathway of this neuropeptide in the spinal cord of rats using capillary size-exclusion liquid chromatography coupled to electrospray ionization mass spectrometry. The applied technique is rapid and selective, and allows for simultaneous measurement and quantitation of several fragments in the incubation mixture.