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Updated: Oct 10, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
Simultaneous micellar electrokinetic chromatographic determination of isomeric fatty acid hydroperoxides and
1Department of Analytical Chemistry. Bergische Universität-Gesamthochschule Wuppertal, Germany.
Abstract:
The high selectivity and efficiency of micellar electrokinetic chromatography with a borax-sodium dodecylsulfate (SDS) or meglumin-SDS buffer make possible the rapid separation of hydroperoxy and hydroxy fatty acids and the non-oxidised unsaturated fatty acids from which they are derived. Nearly all the isomers of the hydroperoxides and hydroxy fatty acids derived from oleic, linoleic, alpha- and gamma-linolenic and arachidonic acids can be determined both qualitatively and quantitatively within ca. 10 min. The system has as many as 1 x 10(6) theoretical plates, and the detection limits with UV diode array detection at 195 or 234 nm are in the micromolar range.
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