Related Experiment Video
Updated: Jul 2, 2026

Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae
Published on: March 10, 2012
Production and purification of recombinant human alpha 2C2 adrenergic receptor using Saccharomyces cerevisiae
A Kapat1, V P Jaakola, H Heimo
1Institute of Biotechnology, University of Helsinki, Finland.
Abstract:
The objective is to generate milligram quantities of recombinant human alpha 2C2 adrenergic receptor for X-ray crystallographic studies. It has been cloned in Saccharomyces cerevisiae, and the production level is at best about 13 pmol/mg of membrane protein, as estimated by radio-ligand binding assay. The receptor is solubilized with sucrose monolaurate followed by immunoaffinity purification and reconstitution into phospholipid vesicles. The efficiency of solubilization and immuno-purification are 60% and 91%, respectively.
Related Concept Videos
Bioreactor Controls-III
Upstream Processing
Production of Alcohol
Production of Pharmaceuticals

