High-speed pectic enzyme fractionation by immobilised metal ion affinity membranes
S A Camperi1, M Grasselli, O Cascone
1Cátedra de Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica, UBA, Argentina. scamperi@huemul.ffyb.uba.ar
Abstract:
Immobilised metal ion affinity polysulfone hollow-fibre membranes, with a high capacity for protein adsorption, were prepared and their utilisation for commercial pectic enzyme fractionation was studied. The pass-through fraction containing pectinlyase is useful for fruit-juice clarification without methanol production on account of pectinesterase being retained by the IDA-Cu2+ membrane.
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