A factorial design analysis of chitin production by Cunninghamella elegans

Chitin production by Cunninghamella elegans (IFM 46109) was studied with a two-level full factorial design, varying time of cultivation and the concentration of D-glucose, L-asparagine, and thiamine in the culture medium. The material extracted was characterized by infrared and NMR spectroscopy. The highest chitin yield, 28.8%, was comparable with the highest in the literature and was obtained with a medium containing 60 g.L-1 of glucose, 3 g.L-1 of asparagine, and 0.008 mg.L-1 of thiamine. Increasing the time of cultivation from 24 h to 72 h did not affect chitin production. The three factors showed significant positive effects on chitin production, without interactions between them.