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Automated bichromatic analysis of serum ceruloplasmin
Annals of Clinical and Laboratory Science
|January 1, 1975
Summary
This study presents an automated method for measuring serum ceruloplasmin (Cp) using p-phenylenediamine oxidase activity. The reliable and accurate automated assay correlates well with manual methods, offering precise Cp concentration measurements.
Area of Science:
- Clinical Chemistry
- Biochemistry
- Analytical Chemistry
Background:
- Ceruloplasmin (Cp) is a key copper-carrying protein in serum.
- Accurate measurement of Cp is crucial for diagnosing various medical conditions.
- Existing manual methods for Cp assay can be time-consuming and labor-intensive.
Purpose of the Study:
- To develop and validate an automated micromethod for serum ceruloplasmin measurement.
- To assess the precision and accuracy of the automated method.
- To compare the automated method with a manual reference procedure.
Main Methods:
- An automated micromethod utilizing the Abbott bichromatic analyzer was developed.
- Serum ceruloplasmin was quantified by measuring its p-phenylenediamine oxidase activity.
- Assay involved mixing serum with p-phenylenediamine substrate and spectrophotometric measurement of product formation after thermal equilibration.
Main Results:
- The automated method demonstrated high precision with within-run CV of 1.1% and day-to-day CV of 3.3%.
- Measurements of ceruloplasmin concentrations in 75 patients showed a strong correlation (r=0.973) with a manual reference method.
- The mean serum ceruloplasmin concentration in healthy men was 29 mg/dL, with 95th percentile limits of 22-40 mg/dL.
Conclusions:
- The described automated micromethod provides a precise and accurate means for serum ceruloplasmin determination.
- This automated assay is a reliable alternative to manual methods for clinical laboratories.
- The method facilitates efficient and accurate assessment of ceruloplasmin levels in patient populations.