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Polyphosphazene microspheres for insulin delivery.

P Caliceti1, F M Veronese, S Lora

  • 1Department of Pharmaceutical Sciences, University of Padua, Via F. Marzolo, 5, 35131, Padova, Italy. caliceti@dsfarm.unipd.it

International Journal of Pharmaceutics
|January 4, 2001
PubMed
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Polyphosphazene microspheres offer potential for insulin delivery. Method B demonstrated sustained glucose reduction in diabetic mice over 1000 hours, outperforming other methods.

Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Polymer Chemistry

Background:

  • Diabetes mellitus requires effective insulin therapy.
  • Controlled insulin release systems are crucial for managing blood glucose levels.
  • Polyphosphazenes are versatile polymers for biomedical applications.

Purpose of the Study:

  • To develop and characterize polyphosphazene-based microspheres for insulin delivery.
  • To evaluate the impact of different preparation methods on microsphere properties and insulin release.
  • To assess the in vivo efficacy and immunogenicity of insulin-loaded microspheres.

Main Methods:

  • Microsphere preparation using suspension-solvent evaporation, double emulsion-solvent evaporation, and combined methods.
  • Scanning electron microscopy (SEM) for morphological analysis.

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  • In vitro insulin release studies and subcutaneous administration in diabetic mice.

    • Main Results:

    • All methods yielded spherical microspheres with porous, honeycomb structures.
    • Methods A and C showed higher protein loading than Method B.
    • Method B microspheres provided sustained glucose reduction in diabetic mice for up to 1000 hours.
    • All formulations induced anti-insulin antibody production over 8 weeks.

    Conclusions:

    • Preparation method significantly influences polyphosphazene microsphere characteristics and performance.
    • Double emulsion-solvent evaporation (Method B) is optimal for long-term insulin delivery, achieving sustained glycemic control.
    • Further investigation into immunogenicity is warranted for clinical translation.