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Updated: Jul 21, 2026

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method
10:36

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

Published on: July 27, 2016

A novel multiple affinity purification tag and its use in identification of proteins associated with a cyclin-CDK

S Honey1, B L Schneider, D M Schieltz

  • 1Cold Spring Harbor Laboratories, Cold Spring Harbor, NY 11724, USA.

Nucleic Acids Research
|February 13, 2001
PubMed

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...