Global expression analysis of extracellular matrix-integrin interactions in monocytes
A R de Fougerolles1, G Chi-Rosso, A Bajardi
1Biogen Inc, Cambridge, Massachusetts 02142, USA. tony_de_fougerolles@biogen.com
Abstract:
Central to immune and inflammatory responses are the integrin-mediated adhesive interactions of cells with their extracellular matrix (ECM)-rich environment. Using a comprehensive and quantitative mRNA profiling technique, we analyzed the effect of ECM-induced attachment on monocyte gene expression, its regulation by growth factors, and the integrin specificity of this event. Adhesion of cells to fibronectin resulted in increased expression of a large number of genes, which was strongly potentiated by the presence of growth factors. Adhesion activated both the NF-kappaB and Jak/STAT pathways of gene transcription and increased expression of genes involved in inflammatory and immune responses, revealing the importance of ECM-integrin interactions in these processes.
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