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Updated: Aug 11, 2026
Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
Published on: April 16, 2014
Effects of ethanol and acetaldehyde on isolated nerve ending membranes: study by atomic-forced microscopy
O V Chumakova1, A V Liopo, S A Chizhik
1Institute of Biochemistry, National Academy of Sciences of Belarus, Grodno. val@biochem.unibel.by
Abstract:
A new method of fixation of native synaptosomes and synaptosomal membranes from rat striatum was applied for their visualization by atomic-force microscopy. A scheme for examination of the surface of biological material was developed, which helps to distinguish intact synaptosomes from washed synaptic membranes and evaluate damage to synaptic membrane surface caused by ethanol (25 mM) and acetaldehyde (50 microM). The proposed method can be used for evaluation of the damaging effects of ethanol and acetaldehyde on neurons.
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