Effect of temperature on guinea pig urinary bladder contraction mediated via P2X-receptors

In vitro experiments showed that P2X-receptor agonist alpha,beta-methylene-ATP and electrical field stimulation in the presence of muscarinic and alpha-adrenoreceptors blockers induced contractile responses of isolated guinea pig bladder, which were more pronounced at 30 degrees C than at 37 degrees C or 42 degrees C. P2X-receptor antagonist pyridoxal-6-phosphate-2',4'-disulfonic acid, produced a more potent inhibitory effect on contractions induced by electrical field stimulation at 30 degrees C in comparison with that at 37 degrees C or 42 degrees C, while the contractions induced by alpha,beta-methylene-ATP were similarly suppressed at all examined temperatures.