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[Culture of human corneal epithelial cells]
1Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Purpose:
To select culture technique of human epithelial cells for grafts.
Methods:
Epithelial cells of limbus were collected by improved enzymatic disaggregation or explant technique growth curves and to calculate doubling time. DNA synthesis of cells were measured with 3H-thymidine incorporation and liquid scintillation techniques.
Results:
Doubling time of cells which were collected by enzymatic disaggregation and by explant technique were 54.15 +/- 4.28 h and 67.88 +/- 1.96 h (P < 0.01). Cellular DNA synthesis of the former was more active (P < 0.01).
Conclusions:
Epithelial cells which were collected by improved enzymatic disaggregation from limbus include stem cells of corneal epithelium. These cells show more active cellulde proliferation and are fitter for use as grafts.