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Related Experiment Videos

[Intra- and intercellular Ca(2+)-signal transduction].

B Himpens1, J Vereecke

  • 1Laboratorium Fysiologie KU-Leuven U.Z. Gasthuisberg Herestraat 49, B 3000 Leuven.

Verhandelingen - Koninklijke Academie Voor Geneeskunde Van Belgie
|February 24, 2001
PubMed
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Cells utilize diverse mechanisms for intracellular and intercellular communication, primarily involving calcium signaling. Mechanical stimulation triggers calcium waves through gap junctions in some cells, while others release extracellular mediators like purinergic agonists.

Area of Science:

  • Cell Biology
  • Biophysics
  • Signaling Pathways

Context:

  • Calcium ions (Ca2+) are crucial second messengers regulating diverse cellular processes, including secretion and differentiation.
  • Multicellular organisms require precise orchestration of cellular functions through intercellular communication (IC).
  • Investigating intracellular and intercellular calcium (Ca2+) wave propagation mechanisms is essential for understanding cellular coordination.

Purpose:

  • To investigate intracellular Ca2+ propagation and intercellular communication mechanisms in various cell types.
  • To elucidate the roles of gap junctions and extracellular mediators in Ca2+ wave transmission.
  • To explore the impact of protein kinase C (PKC) activity and high glucose on intercellular communication.

Summary:

Related Experiment Videos

  • Mechanical stimulation induced intracellular Ca2+ waves in ROS 17/2.8 osteoblasts, RPE, and CPAE cells, propagating to neighboring cells via influx, release from stores, and intercellular pathways.
  • Gap junctions (GJ) mediated IC in RPE and ROS cells, inhibited by heptanol/halothane, with differential PKC modulation; high glucose decreased Cx43 expression and communication in RPE cells.
  • CPAE cells showed negligible GJ contribution to IC; mechanical stimulation triggered Ca2+ waves via extracellular mediators, primarily purinergic agonists like ADP, independent of GJ modulation.

    • Impact:

    • Demonstrates a wide spectrum of cellular communication mechanisms, highlighting cell-type-specific responses to stimuli.
    • Reveals distinct pathways for Ca2+ wave propagation, including gap junction-dependent and independent mechanisms.
    • Provides insights into how factors like mechanical stress and high glucose influence intercellular communication and connexin expression.