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Biomaterial engineered electrodes for bioelectronics.

V Pardo-Yissar1, E Katz, I Willner

  • 1Institute of Chemistry, Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel.

Faraday Discussions
|February 24, 2001
PubMed
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We demonstrate switchable control of electron transfer in cytochrome c (Cyt c) heme proteins immobilized on gold electrodes. This allows for tunable interfacial electron transfer kinetics, enabling applications in bioelectrocatalysis.

Area of Science:

  • Electrochemistry
  • Biophysics
  • Materials Science

Background:

  • Cytochrome c (Cyt c) is a crucial heme protein involved in electron transport.
  • Direct electrical contact with electrodes is essential for bioelectrocatalytic applications.

Purpose of the Study:

  • To investigate the potential-induced switchable control of interfacial electron transfer in single-cysteine mutants of Cyt c.
  • To develop an integrated electrode for oxygen reduction using Cyt c and cytochrome oxidase (COx).

Main Methods:

  • Covalent attachment of single-cysteine Cyt c mutants to maleimide-functionalized gold electrodes.
  • Utilizing double-potential-step chronoamperometry to study electron transfer kinetics.
  • Integrating Cyt c with COx for bioelectrocatalytic oxygen reduction.

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Main Results:

  • The G1C-Cyt c mutant exhibited switchable interfacial electron transfer rates (ket1 = 20 s-1, ket2 = 1.5 s-1) based on electrode potential.
  • Surface attraction and repulsion of Cyt c influenced electron transfer kinetics.
  • An integrated Cyt c/COx electrode demonstrated efficient O2 reduction, with primary electron transfer as the rate-limiting step.

Conclusions:

  • Potential-induced electrostatic interactions can control interfacial electron transfer in immobilized Cyt c.
  • The integrated Cyt c/COx electrode shows promise for bioelectrocatalytic applications.
  • Understanding electron transfer mechanisms is key to optimizing bioelectrocatalytic systems.