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Determination of vitamins A and E in milk samples by fluorescence in micellar media
P Ramos-Lledó1, S Vera, M P San Andrés
1Dpto. Química Analítica, Facultad de Química, Universidad de Alcalá, 28871 Alcalá de Henares, Spain.
Fresenius' Journal of Analytical Chemistry
|February 24, 2001
Abstract:
The fat-soluble vitamins A and E in milk samples were determined by fluorescence at room temperature in an aqueous media of micellar solutions. Different types of surfactants were studied; the cationic hexadecyltrimethylammonium bromide (CTAB), the anionic sodium dodecylsulfate (SDS) and the non-ionic polyoxyethylene(23)laurylether (Brij 35). The detection limits ranged between 50 and 90 ng.L-1 for both vitamins in CTAB and Brij 35. The method has been applied to the determination of vitamins A and E in milk samples.