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[Vibrio cholerae O139 bacteriophages].
T A Kudriakova1, L D Makedonova, G V Kachkina
1Research Institute for Plague Control Microbe, Saratov, Russia.
Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
|February 24, 2001
Summary
Cholera bacteriophages were identified from 27 Vibrio cholerae O139 cultures. These phages, belonging to groups A1 and F1, aid in differentiating V. cholerae O139 strains using prophage typing.
Area of Science:
- Microbiology
- Virology
- Bacteriology
Background:
- Vibrio cholerae O139 is a significant cause of cholera outbreaks.
- Lysogenic V. cholerae strains harbor bacteriophages within their genomes.
- Characterizing these bacteriophages is crucial for understanding bacterial epidemiology.
Purpose of the Study:
- To isolate and characterize cholera bacteriophages from V. cholerae O139.
- To determine the morphological and serological properties of the isolated phages.
- To evaluate the utility of these phages for differentiating V. cholerae O139 strains.
Main Methods:
- Isolation of bacteriophages from 27 lysogenic V. cholerae O139 cultures.
- Morphological classification of phages into groups A1 and F1.
- Serological typing of phages into types II and XII.
- Application of prophage typing and phage sensitivity tests for strain differentiation.
Main Results:
- Successfully isolated bacteriophages from 27 lysogenic V. cholerae O139 cultures.
- Identified phages belonging to morphological groups A1 and F1, and serological types II and XII.
- Demonstrated the effectiveness of prophage typing and phage sensitivity testing in differentiating V. cholerae O139 strains.
Conclusions:
- Cholera bacteriophages from V. cholerae O139 can be classified morphologically and serologically.
- Prophage typing and phage sensitivity testing are valuable tools for epidemiological studies of V. cholerae O139.
- This research contributes to the understanding and control of cholera outbreaks.