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[Comparison between colorimetry and HPLC on the stability test of roxithromycin]
1Division of Pharmaceutics, Shenyang Pharmaceutical University, Shenyang 110015, China.
Aim:
To compare the stability of roxithromycin in solutions of different pH.
Methods:
Roxithromycin solutions of different pH were prepared with water, simulate intestinal fluid (SIF) and simulate gastric fluid (SGF) shown to be the stability of these solutions were tested by colorimetry and HPLC.
Results:
Roxithromycin was stable in water, SGF and SIF determined by colorimetry. However, it was found to be stable only in water and SIF but unstable in SGF as determined by HPLC.
Conclusion:
Roxithromycin is unstable in acidic medium like SGF. The metabolite of roxithromycin showed unfavorable interference on the assay of roxithromycin when colorimetry was used. Colorimetry can not be used for the determination and assay of roxithromycin in acidic solution like SGF.