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Rapid determination of PAHs in soil samples by HPLC with fluorimetric detection following sonication extraction
M N Kayali-Sayadi1, S Rubio-Barroso, C A Díaz-Díaz
1Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Spain.
Abstract:
A rapid method for the determination of PAHs in soil samples based on their extraction with methylene chloride by sonication and subsequent separation by HPLC with fluorimetric detection is proposed. A Hypersil Green PAH column was used with a gradient of acetonitrile/water as the mobile phase, together with a program of nine excitation and emission wavelength pairs. Recoveries were in the range 70-98%, except for acenaphthene and naphthalene, at concentration levels 1.08-442 microg/kg with relative standard deviations in the range 2-15% (n = 4). Total PAHs found in soil samples were in the range 15-282 microg/kg. The results were compared with those obtained by applying the 3540 EPA method for two samples.