Interactions between bovine endothelial cells and Pasteurella multocida: association and invasion

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Invasion of Human Cells by a Bacterial Pathogen
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Invasion of Human Cells by a Bacterial Pathogen

Published on: March 21, 2011

M Galdiero1, L De Martino, U Pagnini

  • 1Dipartimento di Patologia e Sanità Animale, Facoltà di Medicina Veterinaria, Università di Napoli Federico II, Italy. mgaldier@unina.it

Research in Microbiology
|April 3, 2001
PubMed

Insights

Pasteurella multocida invades bovine endothelial cells, with bacterial density and incubation time influencing interaction. Internalized bacteria survive without causing cell death, highlighting potential intracellular roles in Pasteurella infections.

Area of Science:

  • Veterinary Microbiology
  • Cell Biology
  • Infectious Diseases

Background:

  • Pasteurella multocida is a significant pathogen in livestock.
  • Understanding bacterial invasion mechanisms is crucial for controlling infections.
  • The intracellular behavior of P. multocida within host cells remains incompletely understood.

Purpose of the Study:

  • To investigate the adherence and invasion of bovine aortic endothelial cells (BAECs) by Pasteurella multocida.
  • To explore the role of internalized bacteria and their potential for intracellular survival.
  • To determine optimal conditions for P. multocida-BAEC interaction.

Main Methods:

  • Co-incubation of P. multocida with BAEC cultures.
  • Assessment of bacterial adherence and invasion.
  • Evaluation of the effect of cytoskeletal inhibitors (cytochalasin D, colchicine, nocodazole) on invasion.
  • Microscopy to observe bacterial survival and exocytosis.

Main Results:

  • P. multocida effectively adheres to and invades BAECs.
  • Optimal interaction occurs at a 100:1 bacteria-to-cell ratio and 4-hour incubation.
  • Bacterial internalization depends on microfilament and microtubule integrity.
  • Internalized P. multocida do not cause endothelial cell death and can survive intracellularly.

Conclusions:

  • P. multocida invades BAECs via a process dependent on cytoskeletal elements.
  • Internalized P. multocida can survive within endothelial cells and undergo exocytosis.
  • These findings suggest a role for intracellular bacteria in the pathogenesis of Pasteurella infections.

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