Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sample preconcentration by field amplification stacking for microchip-based capillary electrophoresis.

J Lichtenberg1, E Verpoorte, N F de Rooij

  • 1SAMLAB, Institute of Microtechnology, University of Neuchâtel, Switzerland. jan.lichtenberg@unine.ch

Electrophoresis
|April 6, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Studying the intracellular bile acid concentration and toxicity in drug-induced cholestasis: Comprehensive LC-MS/MS analysis with human liver slices.

Toxicology in vitro : an international journal published in association with BIBRA·2025
Same author

Mouse precision-cut liver slices as an ex vivo model to study drug-induced cholestasis.

Archives of toxicology·2022
Same author

Leveraging 3D printing to enhance mass spectrometry: A review.

Analytica chimica acta·2021
Same author

Reinventing (Bio)chemical Analysis with Paper.

Analytical chemistry·2018
Same author

3D-Printed Paper Spray Ionization Cartridge with Integrated Desolvation Feature and Ion Optics.

Analytical chemistry·2017
Same author

Controlled, synchronized actuation of microdroplets by gravity in a superhydrophobic, 3D-printed device.

Analytica chimica acta·2017
Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
See all related articles

Field amplification stacking (FAS) microchips enable longer sample plugs for up to 65-fold signal gains in capillary electrophoresis (CE). Optimized chip design minimizes fluidic effects for improved analyte stacking and separation.

Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Separation Science

Background:

  • Microchip-based capillary electrophoresis (CE) offers miniaturized analytical capabilities.
  • Achieving high sensitivity in CE often requires pre-concentration techniques.
  • Field amplification stacking (FAS) is a promising method for enhancing analyte signals.

Purpose of the Study:

  • To develop and optimize a microchip structure for field amplification stacking (FAS).
  • To investigate and mitigate fluidic effects impacting sample stacking and separation.
  • To enhance signal gains in capillary electrophoresis (CE) through improved chip design.

Main Methods:

  • Development of a novel microchip with integrated field amplification stacking (FAS) capabilities.

Related Experiment Videos

  • Utilized video imaging to observe fluidic phenomena, such as pressure-driven Poiseuille flow.
  • Implemented a coupled-column design with separate stacking and CE channels for full-column stacking and matrix removal via polarity switching.

    • Main Results:

    • Achieved up to 20-fold signal gains with 400 µm long plugs in a 7.5 cm channel using initial FAS design.
    • Observed and characterized pressure-driven Poiseuille flow due to mismatched ionic strengths and electroosmotic velocities.
    • Demonstrated signal enhancements up to 65-fold with a new chip layout featuring a 69 mm stacking channel for full-column stacking.

    Conclusions:

    • Field amplification stacking (FAS) on microchips is effective for generating long sample plugs and achieving significant signal amplification.
    • Understanding and controlling fluidic effects, particularly Poiseuille flow, is crucial for optimizing analyte stacking.
    • The developed coupled-column chip design with polarity switching offers a powerful approach for high-sensitivity CE analysis.