Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

MEK kinase activity is not necessary for Raf-1 function.

M Hüser1, J Luckett, A Chiloeches

  • 1Department of Biochemistry, MRC Toxicology Unit and Division of Biomedical Services, University of Leicester, University Road, Leicester LE1 7RH, UK.

The EMBO Journal
|April 11, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnosis of <i>Mycoplasma columborale</i> in a fledgling kererū (<i>Hemiphaga novaeseelandiae</i>).

New Zealand veterinary journal·2024
Same author

Electrospun Polylactic Acid-Based Fibers Loaded with Multifunctional Antibacterial Biobased Polymers.

ACS applied polymer materials·2023
Same author

PLA and PBAT-Based Electrospun Fibers Functionalized with Antibacterial Bio-Based Polymers.

Macromolecular bioscience·2022
Same author

Learning to implement Smart Healthy Age-Friendly Environments.

Translational medicine @ UniSa·2021
Same author

Comparing the accuracy of lay diagnosis of childhood malaria and pneumonia with that of the revised IMCI guidelines in Nigeria.

Journal of public health (Oxford, England)·2020
Same author

International comparison of civilian violent deaths: a public health approach to reduce gun-related deaths in US youth.

Public health·2019
Same journal

PP2A phosphatase regulates Hippo signalling in dual manner.

The EMBO journal·2026
Same journal

The TPR2 corepressor forms condensates with repressors to fine-tune growth and development in rice.

The EMBO journal·2026
Same journal

SenFlag gene signature identifies senescent cells in mouse and human tissues through a conserved core transcriptional program.

The EMBO journal·2026
Same journal

Scalable phosphotyrosine enrichment with SH2 superbinder enables deep profiling of EGF responses.

The EMBO journal·2026
Same journal

Essential nucleus-apical pole linkage maintains division fidelity during Plasmodium progeny formation.

The EMBO journal·2026
Same journal

From cell atlases to mechanisms: bridging scRNA-seq discovery with in vivo genetics.

The EMBO journal·2026
See all related articles

Raf-1 kinase is crucial for preventing apoptosis during mouse development, but its MEK kinase activity is not essential. Gene targeting revealed distinct roles for Raf-1 beyond cell proliferation.

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Developmental Biology

Background:

  • Raf-1 protein kinase is a key component of the Ras/Raf/MEK/ERK signaling pathway.
  • Raf-1 activation involves RAS:GTP binding and tyrosine phosphorylation at residues 340/341.

Purpose of the Study:

  • To investigate the essential functions of Raf-1 during mouse development.
  • To determine the role of Raf-1's MEK kinase activity in vivo.
  • To elucidate the contribution of Raf-1 to apoptosis and cell proliferation.

Main Methods:

  • Gene targeting in mice to create a raf-1 knockout (Raf-1(-/-)).
  • Generation of a raf-1 mutant mouse with Y340FY341F substitutions (raf-1(FF/FF)).
  • Analysis of embryonic lethality, vascular defects, apoptosis, cell proliferation, and ERK activation.

Related Experiment Videos

Main Results:

  • Raf-1(-/-) mice exhibit embryonic lethality with vascular defects and increased apoptosis.
  • raf-1(FF/FF) mice are viable and fertile, with normal phenotypes and ERK activation.
  • Cells from raf-1(FF/FF) mice show no detectable MEK kinase activity in vitro.

Conclusions:

  • Raf-1's MEK kinase activity is dispensable for normal mouse development.
  • Raf-1 plays a critical role in inhibiting apoptosis during embryogenesis.
  • The study highlights distinct functions of Raf-1 beyond its canonical kinase activity.