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Related Experiment Videos

The TRAP/SMCC/Mediator complex and thyroid hormone receptor function.

M Ito1, R G Roeder

  • 1Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, USA.

Trends in Endocrinology and Metabolism: TEM
|April 18, 2001
PubMed
Summary
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The TRAP/SMCC/Mediator complex, a key transcriptional regulator, involves TRAP220. Its absence impacts thyroid hormone receptor (TR) function and development, but not cell survival.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • The TRAP/SMCC/Mediator complex is a large, conserved mammalian transcriptional regulatory machinery.
  • Initially identified as a thyroid hormone receptor (TR)-associated protein (TRAP) complex, it mediates TR-activated transcription.
  • It functions as a broad coactivator for diverse nuclear receptors and transcription factors.

Purpose of the Study:

  • To investigate the role of the TRAP220 subunit within the TRAP/SMCC/Mediator complex.
  • To determine the in vivo function of TRAP220 in TR-mediated transcription and developmental processes.
  • To assess the necessity of TRAP220 for cell viability.

Main Methods:

  • Genetic ablation of the TRAP220 subunit in murine models.
  • Analysis of TR-mediated transcriptional activity.

Related Experiment Videos

  • Evaluation of developmental and homeostasis events in knockout mice.

    • Main Results:

    • TRAP220 is essential for optimal thyroid hormone receptor (TR) function.
    • Genetic ablation of TRAP220 affects early development and adult homeostasis in mice.
    • TRAP220 is not required for basic cell viability.

    Conclusions:

    • TRAP220 is a critical component of the TRAP/SMCC/Mediator complex, indispensable for TR activity.
    • TRAP220 plays a vital role in developmental and homeostatic processes, highlighting its physiological importance.
    • The findings underscore the specific, non-essential role of TRAP220 in maintaining cell survival.