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Related Experiment Videos

High throughput flow cytometry.

F W Kuckuck1, B S Edwards, L A Sklar

  • 1Cytometry, Cancer Research and Treatment Center, University of New Mexico Health Sciences Center, Albuquerque, New Mexico.

Cytometry
|April 20, 2001
PubMed
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A new method significantly increases flow cytometry throughput, enabling rapid analysis of multiple samples for drug discovery. This advance allows for over 100,000 samples per day, meeting high-throughput demands.

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Cell Biology

Background:

  • Conventional flow cytometry is limited in analyzing multiple samples rapidly.
  • High-throughput screening in drug discovery requires analyzing up to 100,000 samples daily.
  • Current limitations hinder the application of flow cytometry in high-throughput drug discovery.

Purpose of the Study:

  • To develop a method for significantly increasing flow cytometry throughput.
  • To enable rapid analysis of multiple samples using flow cytometry.
  • To meet the high-throughput demands of drug discovery.

Main Methods:

  • Connecting commercial peristaltic tubing from an autosampler to a flow cytometer.
  • Delivering samples via a peristaltic pump from multiwell plates, separated by air bubbles.

Related Experiment Videos

  • Validating the approach with instruments from three different manufacturers.

    • Main Results:

    • Achieved throughput rates approaching the autosampler limit (up to 100 wells per minute).
    • Maintained particle integrity within light scatter and fluorescence gates.
    • Demonstrated low inter-well carryover (<5% without wash, <1% with wash) at the microliter scale.

    Conclusions:

    • The described method enables flow cytometry throughput of 100,000+ samples per day.
    • This approach is well-suited for end-point assays.
    • Potential for nearly 1 billion assays per day when combined with high-speed sorting and single-cell assays.