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Published on: November 12, 2016
Quantitative HPLC analysis of acids in Brazilian cachaças and various spirits using fluorescence detection of their
R Ferreira Do Nascimento1, D Rodrigues Cardoso, D De Keukeleire
1Instituto de Química de São Carlos, Departamento de Química e Física Molecular, Universidade de São Paulo, Caixa Postal 780, 13560-970 São Carlos, SP, Brazil.
Abstract:
An analytical procedure for the separation and quantification of 17 short-chain, medium-chain, and long-chain acids in cachaças and various spirits has been developed involving C18 solid phase extraction, derivatization with 9-anthryldiazomethane, and reverse phase HPLC using fluorescence detection. The limit of detection was between 5 and 15 fmol, whereas the recovery of nonanoic acid as internal standard was >95%. Relative standard deviation values for reproducibility were between 0.09 and 20.4%, and repeatability was between 0.05 and 11.3%.
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