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Human Taf(II)130 is a coactivator for NFATp
L J Kim1, A G Seto, T N Nguyen
1Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, Colorado 80309-0215, USA.
Molecular and Cellular Biology
|April 21, 2001
Summary
Human TAF(II)130 acts as a coactivator for NFATp, a transcription factor regulating cytokine genes. This study biochemically characterizes NFATp
Area of Science:
- Molecular Biology
- Gene Regulation
- Biochemistry
Background:
- NFATp is a transcription factor crucial for cytokine gene expression.
- Understanding NFATp's transcriptional activation mechanisms is vital.
Purpose of the Study:
- To elucidate the molecular mechanisms of NFATp-mediated transcriptional activation.
- To identify coactivators involved in NFATp function.
Main Methods:
- Established a reconstituted human transcription system.
- Performed in vitro interaction assays.
- Utilized overexpression studies in Cos-1 cells.
Main Results:
- TFIID complex, specifically hTAF(II)130, is required for NFATp activation.
- hTAF(II)130 directly binds to the C-terminal domain of NFATp.
- A partial TFIID complex containing hTAF(II)130 supported NFATp-activated transcription in vitro.
- Overexpression of hTAF(II)130 inhibited NFATp activity.
Conclusions:
- hTAF(II)130 functions as a coactivator for NFATp.
- This study provides the first biochemical characterization of NFATp transcriptional activation.