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Related Experiment Videos

Gal repressosome contains an antiparallel DNA loop.

M Geanacopoulos1, G Vasmatzis, V B Zhurkin

  • 1Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.

Nature Structural Biology
|April 27, 2001
PubMed
Summary

Gal repressor (GalR) tetramer formation and DNA looping in E. coli were studied. The findings reveal a preferred antiparallel DNA loop orientation for gal operon repression.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Structural Biology

Background:

  • Gal operon regulation in Escherichia coli involves the GalR repressor and histone-like HU protein.
  • Repressosome assembly and DNA looping are critical for gene repression.

Purpose of the Study:

  • To elucidate the structural basis of GalR tetramer formation.
  • To model and evaluate potential DNA loop configurations during gal operon repression.

Main Methods:

  • Structure-based genetic analysis to identify GalR interaction surfaces.
  • Construction of stereochemical models for DNA loops.
  • Evaluation of DNA elastic energies.

Main Results:

  • Defined GalR surfaces mediating tetramer formation into a stacked, V-shaped structure.

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  • Generated models for four DNA loop possibilities.
  • Identified an antiparallel orientation of gal operators as the preferred DNA loop structure, causing DNA undertwisting.

    • Conclusions:

    • The V-shaped GalR tetramer and antiparallel DNA loop orientation are key to gal operon repression.
    • DNA elastic energy dictates the preferred loop conformation.