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Apical localization of wingless transcripts is required for wingless signaling.
A J Simmonds1, G dosSantos, I Livne-Bar
1Banting and Best Department of Medical Research, University of Toronto, Room 312, Charles H. Best Institute, 112 College Street, Ontario, Toronto, Canada.
Cell
|May 5, 2001
Summary
The location of Wingless (Wnt) mRNA dictates Wingless protein distribution and function in epithelial cells. This mRNA localization mechanism is conserved and crucial for establishing cell polarity.
Area of Science:
- Cell Biology
- Developmental Biology
- Molecular Biology
Background:
- Polarized epithelia are fundamental to developing and adult tissues.
- Protein trafficking is the presumed mechanism for asymmetric protein distribution in epithelial cells.
Purpose of the Study:
- To investigate the role of mRNA localization in the distribution and function of the Wingless signaling protein.
- To determine the mechanism underlying Wingless mRNA apical localization.
Main Methods:
- High-resolution in situ hybridization to visualize mRNA distribution.
- 3' UTR sequence analysis to identify localization elements.
- Functional assays to assess protein distribution and signaling activity.
Main Results:
- Wingless mRNA exhibits apical localization in most examined tissues.
- Two independent elements in the 3' UTR mediate this apical localization.
- Altering these elements changed protein distribution and reduced signaling activity.
Conclusions:
- Subcellular mRNA localization is a key determinant of Wingless protein distribution and function.
- This mechanism is conserved and likely plays a common role in establishing epithelial cell polarity.
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