Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

FLASH coordinates NF-kappa B activity via TRAF2.

Y H Choi1, K B Kim, H H Kim

  • 1Department of Life Science, Kwangju Institute of Science and Technology, Puk-gu, Kwangju 500-712, Korea.

The Journal of Biological Chemistry
|May 8, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Observation of Excitonic Instability in a Monolayer Ta<sub>2</sub>NiSe<sub>5</sub> With Strain Disorder.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Intertwined orders in a quantum-entangled metal.

Nature materials·2026
Same author

Training Tomorrow's Leaders in Cancer Immunology.

Cancer immunology research·2026
Same author

Bidirectional Ultrafast Control of Charge Density Waves via Phase Competition.

Physical review letters·2026
Same author

Validation of a novel automated Alberta Stroke Program Early Computed Tomography Score (ASPECTS) software: reliability, magnetic resonance imaging (MRI) correlation, and early ischaemic core discrimination.

Clinical radiology·2025
Same author

Quantum interference and occupation control in high harmonic generation from monolayer WS<sub>2</sub>.

Nature communications·2025

The FLASH protein regulates the NF-kappaB pathway activation, crucial for tumor necrosis factor-alpha (TNF-alpha) signaling. Inhibiting FLASH blocks TNF-alpha-induced NF-kappaB activation, while its overexpression enhances it via TRAF2.

Area of Science:

  • Cellular biology
  • Molecular signaling
  • Immunology

Background:

  • The FLASH protein interacts with caspase-8 and is involved in apoptosis.
  • Tumor necrosis factor-alpha (TNF-alpha) signaling activates the NF-kappaB pathway.
  • Understanding FLASH's role in TNF-alpha signaling is crucial for cellular regulation.

Purpose of the Study:

  • To investigate the role of FLASH in TNF-alpha-induced NF-kappaB activation.
  • To elucidate the molecular mechanism by which FLASH influences NF-kappaB signaling.
  • To identify interacting partners of FLASH in the TNF-alpha pathway.

Main Methods:

  • Antisense oligonucleotide-induced inhibition of FLASH expression.
  • Overexpression of FLASH and dominant-negative mutants.

Related Experiment Videos

  • Luciferase reporter gene assay for NF-kappaB activity.
  • Co-immunoprecipitation and in vitro binding assays.

    • Main Results:

    • FLASH inhibition abolished TNF-alpha-induced NF-kappaB activation.
    • FLASH overexpression dose-dependently activated NF-kappaB.
    • FLASH directly interacts with TRAF2, a key signaling intermediate.
    • A specific domain of FLASH (residues 856-1191) mediates NF-kappaB activation and TRAF2 binding.

    Conclusions:

    • FLASH plays a significant role in coordinating NF-kappaB activity downstream of TNF-alpha.
    • The FLASH-TRAF2 interaction is critical for this regulation.
    • FLASH acts as a molecular link in the TNF-alpha signaling pathway, influencing NF-kappaB activation via TRAF2.