Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Modulation of epithelial tissue and cell migration by microgrooves.

B A Dalton1, X F Walboomers, M Dziegielewski

  • 1CSIRO Molecular Science, P.O. Box 184, North Ryde, New South Wales 1670, Australia. ann.dalton@molsci.csiro.au

Journal of Biomedical Materials Research
|May 8, 2001
PubMed
Summary

Surface microgrooves guide epithelial tissue migration. Deeper microgrooves significantly influenced cell movement direction and adhesion compared to groove width variations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Third-generation modular mandible endoprosthesis in Macaca fascicularis.

International journal of oral and maxillofacial surgery·2020
Same author

Calcium phosphate cements: Optimization toward biodegradability.

Acta biomaterialia·2020
Same author

Vitamin D Deficiency Is Associated With Longer Hospital Stay And Lower Functional outcome After Total Knee Arthroplasty.

Acta orthopaedica Belgica·2018
Same author

A Novel Thermoresponsive Gel as a Potential Delivery System for Lipoxin.

Journal of dental research·2018
Same author

Use of programme budgeting and marginal analysis to set priorities for local NHS dental services: learning from the north east of England.

Journal of public health (Oxford, England)·2018
Same author

Spheroid formation and stemness preservation of human periodontal ligament cells on chitosan films.

Oral diseases·2018

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Tissue Engineering

Background:

  • Epithelial tissue regeneration is crucial for wound healing and biomaterial integration.
  • Understanding cell migration on microstructured surfaces is key to designing effective biomedical implants.

Purpose of the Study:

  • To investigate the influence of microgroove dimensions (depth and width) on epithelial cell and tissue migration.
  • To elucidate the cellular mechanisms underlying guided migration on microgrooved polystyrene substrata.

Main Methods:

  • Utilized polystyrene substrata with precisely fabricated microgrooves (1-10 microm width, 1-5 microm depth).
  • Studied the migration patterns of both intact epithelial tissue sheets and confluent epithelial cell monolayers.
  • Employed scanning electron microscopy (SEM) to visualize cellular extensions and actin filament organization.

Related Experiment Videos

Main Results:

  • Epithelial migration was enhanced along microgrooves and inhibited across them.
  • Microgroove depth had a more significant impact on migration than groove/ridge width.
  • Basal cell layers conformed to microgroove contours, with actin filaments aligning along the grooves.

Conclusions:

  • Surface microgrooves can effectively direct the migration of adjacent epithelial tissue.
  • This directional guidance has significant implications for controlling epithelial tissue organization on implanted biomaterials.