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Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
Published on: May 18, 2011
Urine analysis by laser Raman spectroscopy
W R Premasiri1, R H Clarke, M E Womble
1Chemistry Department, Boston University, Boston, Massachusetts, Boston, MA 02215, USA.
Background And Objective:
We examine the use of Raman spectroscopy to analyze components in human urine.
Study Design/Materials And Methods:
Targeted urine components include urea, uric acid, and creatinine.
Results:
Urea concentration in urine is sufficiently high that normal Raman spectroscopy may be used for its analysis. All other components are in low concentrations requiring the use of surface-enhanced Raman spectroscopic methods (SERS).
Conclusions:
Both normal Raman and SERS approaches have been investigated for total urine-nitrogen determination, urine urea-nitrogen determination, and for the urea/creatinine excretion ratio.
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