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Updated: Jul 10, 2026

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High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
Published on: June 1, 2014
Staphylococcus aureus cell extract transcription-translation assay: firefly luciferase reporter system for evaluating
R W Murray1, E P Melchior, J C Hagadorn
1Pharmacia Corporation, Kalamazoo, Michigan 49007, USA.
Antimicrobial Agents and Chemotherapy
|May 17, 2001
Abstract:
The promoter for the Staphylococcus aureus capsule polysaccharide synthesis gene (cap1A) was cloned in front of the firefly luciferase gene for use in a cell extract S. aureus transcription-translation system. The assay is rapid, reproducible, and sensitive and has a lower background level than the radiolabeled amino acid incorporation translation assays. We present data evaluating a transcription inhibitor and a number of protein translation inhibitors in this system.

