Related Experiment Video
Updated: Aug 13, 2026
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Published on: November 1, 2018
Quantitation of viral DNA by real-time PCR applying duplex amplification, internal standardization, and two-color
F Gruber1, F G Falkner, F Dorner
1Hyland-Immuno Division, Biomedical Research Center, Baxter, A-2304 Orth-Donau, Austria.
Abstract:
A real-time PCR method was developed to quantitate viral DNA that includes duplex amplification, internal standardization, and two-color fluorescence detection without the need to generate an external standardization curve. Applied to human parvovirus B19 DNA, the linear range was from 10(2) to at least 5 x 10(6) copies per ml of sample. The coefficient of variation was 0.29 using a run control of 2,876 copies per ml. The method reduces the risk of false-negative results, yields high precision, and is applicable for other DNA targets.
