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Resolution of cis-trans ambiguities between HLA-DRB1 alleles using single-strand conformation polymorphisms and
1Allogen Laboratories, The Cleveland Clinic Foundation, Cleveland, Ohio, USA. pp@tt.ccf.org
Tissue Antigens
|May 31, 2001
Abstract:
DNA-based typing of HLA alleles occasionally results in the inability to assign a specific allele because of ambiguity in associating two or more polymorphisms to the same or to alternate homologs (cis/trans ambiguity). Since most individuals are heterozygous at a given HLA locus, the highest level of confidence in definition is obtained when the alleles are tested in isolation. By using single-strand conformation polymorphisms (SSCP) to separate heterozygous HLA-DRB1 alleles, followed by sequencing of separated conformers, we have achieved resolution of previously ambiguous assignments without the need for additional probes or primers.