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Published on: September 19, 2018
New configuration in capillary isotachophoresis-capillary zone electrophoresis coupling
1Department of Food Preservation and Meat Technology, Institute of Chemical Technology, Prague, Czech Republic. frantisek.kvasnicka@vscht.cz
Abstract:
The paper surveys possible configurations of a coupling capillary column operating in various electromigration modes. Special attention is given to capillary isotachophoresis-capillary zone electrophoresis (cITP-CZE) coupling and its description from the theoretical point of view. Computer simulations of separation are presented and compared with experiments. Further, we propose a new configuration of electrolyte systems in cITP-CZE coupling, which offers a possibility to perform complex analyses of micro- and macro-constituents in one run. The electrolyte system is verified by practical experiments for both anionic and cationic modes of analysis. The advantages and disadvantages of the new combination are discussed.
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