Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Simian virus 40 large tumor antigen modulates the Raf signaling pathway.

N Grammatikakis1, K Jaronczyk, A Siganou

  • 1Department of Physiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.

The Journal of Biological Chemistry
|May 31, 2001
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dermatologic immune-related adverse events associated with immune checkpoint inhibitors: A prospective real-world observational study in Switzerland.

JAAD international·2026
Same author

Correction: PIK3CA<sup>H1047R</sup>- and Her2-initiated mammary tumors escape PI3K dependency by compensatory activation of MEK-ERK signaling.

Oncogene·2025
Same author

Canadian Surgery Forum: Abstracts of presentations to the Annual Meetings of the Canadian Association of Bariatric Physicians and Surgeons, Canadian Association of General Surgeons, Canadian Association of Thoracic Surgeons, Canadian Hepato-Pancreato-Biliary Association, Canadian Society of Surgical Oncology, Canadian Society of Colon and Rectal Surgeons, Vancouver, BC, Sept. 17-21, 2013.

Canadian journal of surgery. Journal canadien de chirurgie·2025
Same author

Durable benefit from poly(ADP-ribose) polymerase inhibitors in metastatic prostate cancer in routine practice: biomarker associations and implications for optimal clinical next-generation sequencing testing.

ESMO open·2024
Same author

Nivolumab-induced eosinophilic fasciitis: An unusual immune-related adverse event that needs to be recognized by practitioners.

Annales de dermatologie et de venereologie·2023
Same author

Abstracts of presentations to the Annual Meetings of the Canadian Society of Colon and Rectal Surgeons Canadian Association of General Surgeons Canadian Association of Thoracic Surgeons: Canadian Surgery Forum, Toronto, Ont., September 6-9, 2007.

Canadian journal of surgery. Journal canadien de chirurgie·2023

Simian virus 40 large tumor antigen (SVLT) activates Raf kinase, a key signaling molecule. This activation may occur independently of Ras, suggesting a novel oncogenic mechanism for SVLT.

Area of Science:

  • Oncology
  • Molecular Biology
  • Virology

Background:

  • Simian virus 40 large tumor antigen (SVLT) is a known oncogene.
  • SVLT's transforming properties are linked to p53 and pRb inactivation.
  • Previous studies suggested Ras activation in SVLT-expressing cells.

Purpose of the Study:

  • To elucidate the mechanism of action of SVLT.
  • To identify additional molecular targets of SVLT.
  • To investigate the relationship between SVLT, Ras, and Raf kinase.

Main Methods:

  • Expression of SVLT in insect and mammalian cells.
  • Analysis of Raf kinase activity.
  • Use of antisense constructs to reduce Ras levels.

Related Experiment Videos

Main Results:

  • SVLT expression leads to increased Raf kinase activity.
  • SVLT activates Raf kinase in both insect and mammalian cell systems.
  • Raf activation by SVLT persists even when Ras levels are significantly reduced.

Conclusions:

  • Raf kinase is a direct or indirect target of SVLT.
  • SVLT activates Raf kinase through a mechanism partially independent of Ras.
  • This finding reveals a novel pathway in SVLT-mediated oncogenesis.