[The method for detection of urinary lysozyme]

Area of Science:

• Biochemistry
• Analytical Chemistry

Context:

• Lysozyme is a crucial enzyme found in various bodily fluids.
• Accurate measurement of urinary lysozyme is important for diagnosing certain medical conditions.

Purpose:

• To develop and validate a new method for quantifying lysozyme in human urine.
• To establish reference ranges for urinary lysozyme in healthy populations.
• To present preliminary urinary lysozyme data in the context of specific diseases.

Summary:

• A new assay utilizes the adsorption of lysozyme onto chitin surfaces for its measurement.
• The method was applied to urine samples from healthy children, adult men, and adult women.
• Normal urinary lysozyme concentrations were determined to be 1.76 mg/L, 1.51 mg/L, and 1.69 mg/L, respectively.

Impact:

• Provides a reliable method for assessing urinary lysozyme.
• Contributes to understanding normal lysozyme levels in urine.
• Offers potential for improved diagnostics related to diseases affecting urinary lysozyme levels.