Inhibitory and excitatory effects of CNS depressants on invertebrate synapses

(1) The effects of pentobarbital were studied on the membrane properties and synaptic activity of crustacean neuromuscular junction preparations and molluscan neurons. (2) Pentobarbital selectivity depressed in a dose-dependent, reversible manner the exciatory postynaptic potentials (EPSPs) recorded at crustacean neuromuscular junctions without altering either inhibitory postsynaptic potentials (IPSPs) or post-synaptic membrane properties. (3) Pentobarbital depressed cholinergic EPSPs recorded in an identified molluscan neuron and depressed the depolarizing phase of biphasic PSP without affecting the hyperpolarizing phase of the BPSP on the same cell. Facilitation of the EPSP was not affected. (4) Pentobarbital did not appreciably alter the reversal potentials of the EPSP and IPSP. (5) Low concentrations of pentobarbital did not alter the appearance of spontaneously occurring IPSPs, while high concentrations changed the pattern of regular IPSP input to an irregular, burst-like pattern. (6) Pentobarbital and 5 other CNS depressants (cholralose, chloroform, ethanol, and urethane) increased the excitability and altered the current--voltage relations of a cell whose membrane properties have been proposed as a model of presynaptic terminal membranes. The effects were dependent on the species of external divalent cation present. (7) The results in these invertebrate systems may provide insight into the cellular basis of the depressant and excitatory effects of these agents.