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Integrin expression, enterocyte maturation, and bacterial internalization.

D J Hess1, M J Henry-Stanley, E A Moore

  • 1Department of Surgery, University of Minnesota, 420 Delaware St. SE, Minneapolis, MN 55455, USA. hessx006@tc.umn.edu

The Journal of Surgical Research
|June 9, 2001
PubMed
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Bacterial internalization by enterocytes is not linked to integrin expression or differentiation. Instead, exposing the lateral enterocyte membrane appears to be key for bacterial entry.

Area of Science:

  • Microbiology
  • Cell Biology
  • Gastroenterology

Background:

  • Mechanisms of enteric bacteria translocation are poorly understood.
  • Adhesion molecules link enteric pathogens to host cells, but not typically normal enteric bacteria to enterocytes.
  • Enteric pathogen studies suggest links between adhesion molecules, bacterial internalization, and enterocyte differentiation.

Purpose of the Study:

  • Investigate integrin expression and differentiation in enterocytes.
  • Determine enterocyte internalization of Salmonella typhimurium, Proteus mirabilis, and Escherichia coli.

Main Methods:

  • Quantified integrin subunit expression (alpha2, alpha3, beta1) on Caco-2 and HT-29 enterocytes using ELISA.
  • Observed bacteria-enterocyte interactions via light and scanning electron microscopy.

Related Experiment Videos

  • Measured bacterial internalization using the gentamicin protection assay.

    • Main Results:

    • Immature Caco-2 enterocytes showed increased alpha2, alpha3, and beta1 integrin subunit expression compared to mature cells.
    • Immature HT-29 enterocytes had similar alpha3 and beta1 but decreased alpha2 expression versus mature cells.
    • Bacterial internalization increased in immature enterocytes and in mature enterocytes with exposed lateral membranes, but not significantly different between immature and exposed mature enterocytes.

    Conclusions:

    • Integrin expression and enterocyte differentiation do not appear to drive bacterial internalization.
    • Exposure of the lateral enterocyte membrane is a significant factor facilitating bacterial entry into enterocytes.