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Automated solid-phase extraction for sample preparation followed by high-performance liquid chromatography with diode
C Domínguez1, D A Guillén, C G Barroso
1Departamento de Química Analítica, Facultad de Ciencias, Universidad de Cádiz, Puerto Real, Spain.
Abstract:
A method has been developed for the simultaneous determination of resveratrol in all its forms (free isomers and glycosylates) in wines by high-performance liquid chromatography with diode array and mass spectrometric detection. Prior to injection into the column, preconcentration of the sample by automated solid-phase extraction is carried out. In the detection by UV absorption, quantitation was carried out at 280 and 305 nm, and in detection by mass spectrometry, quantitation was performed in the selected ion monitoring mode at m/z 228 and at m/z 238. A comparative study between both detection systems was carried out.